21.15109/CONCORDA/ITBQD4 Fiáth, Richárd0000-0001-8732-2691(HUN-REN Természettudományi Kutatóközpont)Rácz, Melinda0000-0002-6166-7699(HUN-REN Természettudományi Kutatóközpont)Horváth, Csaba0000-0001-8176-8975(HUN-REN Természettudományi Kutatóközpont)Ulbert, István0000-0001-9941-9159(HUN-REN Természettudományi Kutatóközpont)Wittner, Lucia0000-0001-6800-0953(HUN-REN Természettudományi Kutatóközpont) ARP 2023 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Here, we deposited a calcium imaging dataset collected within the framework of the HYPERSTIM Horizon Europe project. The in vivo dataset was obtained from the primary visual cortex of GCaMP6 transgenic mice during electrical microstimulation using a two-photon laser scanning microscope. A subset of the dataset contains spontaneous cortical activity without electrical stimulation (Mouse1 and Mouse2). Mice were anesthetized with ketamine/xylazine. A Femtonics Femto2D two-photon microscope setup was used for data acquisition. A 20x water immersion objective with the following parameters was used for calcium imaging. Field of view (FOV): 550 μm x 550 μm; Numerical aperture (NA): 1.0; Working distance: 2.0 mm. We performed raster scanning with a resonant scanner at ~31 Hz frame rate and with a frame size of 512 x 512 pixels. The wavelength used for calcium imaging was 900 nm. Further details can be found below (see Notes). Fiáth, Richárd(ELKH Természettudományi Kutatóközpont)