In vivo two-photon calcium imaging dataset obtained from the primary visual cortex of GCaMP6 mice during electrical microstimulationhttps://hdl.handle.net/21.15109/CONCORDA/ITBQD4Fiáth, RichárdRácz, MelindaHorváth, CsabaUlbert, IstvánWittner, LuciaARP2023-12-112023-12-11T19:59:22ZHere, we deposited a calcium imaging dataset collected within the framework of the <a href="https://hyperstim.eu/">HYPERSTIM</a> Horizon Europe project. The in vivo dataset was obtained from the primary visual cortex of GCaMP6 transgenic mice during electrical microstimulation using a two-photon laser scanning microscope. A subset of the dataset contains spontaneous cortical activity without electrical stimulation (Mouse1 and Mouse2). Mice were anesthetized with ketamine/xylazine. A Femtonics Femto2D two-photon microscope setup was used for data acquisition. A 20x water immersion objective with the following parameters was used for calcium imaging. Field of view (FOV): 550 μm x 550 μm; Numerical aperture (NA): 1.0; Working distance: 2.0 mm. We performed raster scanning with a resonant scanner at ~31 Hz frame rate and with a frame size of 512 x 512 pixels. The wavelength used for calcium imaging was 900 nm. Further details can be found below (see Notes).Medicine, Health and Life Sciencescalcium imagingelectrical microstimulationtwo-photon microscopyvisual cortexGCaMP6 miceEnglish2023-12-11Fiáth, RichárdHorváth, Csaba; Rácz, Melinda; Fiáth, RichárdWittner, Lucia; Ulbert, István; Fiáth, RichárdHorváth, Csaba; Rácz, Melinda; Fiáth, Richárd; Wittner, Lucia; Ulbert, IstvánIntegrative Neurosciene Research GroupIntegrative Neurosciene Research Group2023-10-03experimental data (calcium imaging)CC0 1.0