Phylogenetic dataset and supplementary material for Surján et al. 2024. Eurasian beaver polyomavirushdl:21.15109/ARP/XV28NNARP2025-11-121Surján, András; Harrach, Balázs; Vidovszky, Márton, 2025, "Phylogenetic dataset and supplementary material for Surján et al. 2024. Eurasian beaver polyomavirus", https://hdl.handle.net/21.15109/ARP/XV28NN, ARP, V1Phylogenetic dataset and supplementary material for Surján et al. 2024. Eurasian beaver polyomavirushdl:21.15109/ARP/XV28NNSurján, AndrásHarrach, BalázsVidovszky, MártonARPSurján, AndrásSurján, András2025-11-06Agricultural Sciencespolyomavirus, novel species, complete genome, Eurasian beaver, phylogenetics, co-evolutionThe Eurasian beaver (Castor fiber), native to Hungary, faced local extinction in 1865 and was successfully reintroduced between mid-1980s and 2008. Despite screening programs focusing on animal health during reintroduction in other countries, information about viruses in the Hungarian beaver population remains limited. Polyomaviruses (PyVs) have been identified in various rodents, and have been detected just recently in beavers by us. In this paper we present the full genome analysis of the first PyV detected in Eurasian beaver. The novel PyV was discovered in the kidney tissues of two specimens. The genome is 5244 bp, and contains four genes. Small T-antigen (STAg) and alternative large T ORF (ALTO) genes are directly fused together forming the middle T-antigen (MTAg). VP3 is absent from the genome. Its large T-antigen (LTAg) coding sequence exhibited over 15% genetic divergence from known PyVs, supporting its classification into a new species within the genus Alphapolyomavirus, suggesting to be named Alphapolyomavirus castoris. Phylogenetic analysis, based on the LTAg gene showed, that the beaver PyV forms a distinct clade with primate PyVs within the genus Alphapolyomavirus, separate from other rodent PyVs. Phylogenetic study of the VP1 gene however showed this virus to belong in a distinct clade with the same primate PyVs, and additionally PyVs from rodents and a myocastor, which suggest host virus co-evolution. The virus detection of the euthanized beavers suggests an apathogenic persistent infections. The aquatic lifestyle of beavers may influence virus transmission, warranting further exploration of undiscovered viruses in beavers.Complete genome characterization and phylogenetic analysis of a novel polyomavirus detected in Eurasian beavers (Castor fiber)10.1016/j.meegid.2024.105620Complete genome characterization and phylogenetic analysis of a novel polyomavirus detected in Eurasian beavers (Castor fiber)FigS1.pngPhylogenetic tree based on the nucleotide sequences of entire VP1 genes.image/pngFigureS1_Description.txtFigure description for supplementary figure 1 (FigureS1).text/plainLTAg_eins.fastaMultiple alignment of LTAg (Large Tumor Antigen) gene nucleotide sequences of polyomaviruses used for phylogenetic analysis, including the the Castor fiber polyomavirus 1, using MAFFT 7.49 with the E-INS-i algorithm.application/octet-streamLTAg_seq.fastaICTV Report on the family: Polyomaviridae resource file, alignment file used for the inference of the phylogenetic tree seen in the report, augmented with the Castor fiber polyomavirus 1 LTAg (Large Tumor Antigen) gene nucleotide sequence.application/octet-streamLTAg_tree.nwkPhylogenetic tree reconstruction results in Newick format, based on LTAg (Large Tumor Antigen) gene sequences, using PhyML 3.3.1, with GTR+G+I model, SPR for topology and SH-like aLRT method for branch support.application/octet-streamRead_me.txtInformation file.text/plainVP1_fftnsi.fastaMultiple alignment of VP1 (major capsid protein) gene nucleotide sequences of polyomaviruses used for phylogenetic analysis, using MAFFT 7.49 with the FFT-NS-i algorithm.application/octet-streamVP1_seq.fastaRaw VP1 (major capsid protein) gene nucleotide sequences of polyomaviruses used for phylogenetic analysis.application/octet-streamVP1_tree.nwkPhylogenetic tree reconstruction results in Newick format, based on VP1 (major capsid protein) gene sequences, using PhyML 3.3.1, with GTR+G+I model, SPR for topology and SH-like aLRT method for branch support.application/octet-stream