Supplementary data – Antimicrobial resistance of Escherichia coli in Hungarian wild rats – Szmolka, 2026hdl:21.15109/ARP/U0YVYVARP2026-06-081Szmolka, Ama; Locsmándi, Gabriella; Makó, Anita; Kiss, Adél; Gellért, Ákos; Egyed, László, 2026, "Supplementary data – Antimicrobial resistance of Escherichia coli in Hungarian wild rats – Szmolka, 2026", https://hdl.handle.net/21.15109/ARP/U0YVYV, ARP, V1Supplementary data – Antimicrobial resistance of Escherichia coli in Hungarian wild rats – Szmolka, 2026hdl:21.15109/ARP/U0YVYVSzmolka, AmaLocsmándi, GabriellaMakó, AnitaKiss, AdélGellért, ÁkosEgyed, LászlóK137798TKP2021-EGA-01ARPSzmolka, AmaKaján, Győző László2026-06-08Agricultural SciencesCTX-M-1 plasmidCore genome MLSTPlasmid replicon typeEscherichia coliRatST17982This study presents a comprehensive analysis of antimicrobial resistance in E. coli from brown rats (Rattus norvegicus), focusing on the occurrence and genetic basis of resistance and characterizing an ESBL-producing E. coli strain with a fully resolved CTX-M-1 plasmid. 25.6% of 90 brown rats carried AMR E. coli strains, with 8.9% displaying multidrug resistance. The predominant resistance pattern was combined resistance to ampicillin (17.8%) and tetracycline (12%), with plasmid-associated resistance genes blaTEM−1 and tet(A)/tet(B). The study identified the first ESBL-producing E. coli strain (88/Ec2) in an urban rat in Hungary, harboring the gene blaCTX−M−1 on an approximately 92 kb IncI1 plasmid pCTX-M-1_88/Ec2. Comparative plasmid analysis showed 98% structural similarity to CTX-M-1 plasmids from human-derived pathogens. Strain 88/Ec2 was serotyped as O168:H38 and assigned to a novel sequence type, ST17982. Phylogenetically, it exhibits a central relationship to human CTX-M E. coli lineages, suggesting a shared genomic background with globally disseminated, human epidemic E. coli lineages. These findings highlight the role of urban brown rats as reservoirs and potential vectors of antimicrobial resistance within the One Health framework and advocate for enhanced surveillance of AMR in urban wildlife to better understand and mitigate the zoonotic transfer of resistance genes.20212024Hungary<a href="http://creativecommons.org/licenses/by-nc/4.0">CC BY-NC 4.0</a>The datasets generated for this study are available in BioProject PRJNA1303976.Szmolka, A., Locsmándi, G., Makó, A. et al. Antimicrobial resistance of Escherichia coli in Hungarian wild rats and characterization of a CTX-M-1 type ESBL plasmid. Sci Rep 16, 8583 (2026)10.1038/s41598-026-38191-6Szmolka, A., Locsmándi, G., Makó, A. et al. Antimicrobial resistance of Escherichia coli in Hungarian wild rats and characterization of a CTX-M-1 type ESBL plasmid. Sci Rep 16, 8583 (2026)Figure_S1.pdfNumber of cohabiting AMR *E. coli* strains with the indicated AMR traits in the 23 intestinal 10 samples from brown rats (*Rattus norvegicus*)application/pdfFigure_S1.pngNumber of cohabiting AMR *E. coli* strains with the indicated AMR traits in the 23 intestinal 10 samples from brown rats (*Rattus norvegicus*)image/pngreadme.mdreadme file in md formattext/markdownTable_S1.csvqPCR primer and probe sequences for antimicrobial resistance gene detection in *E. coli*text/csvTable_S1.xlsxqPCR primer and probe sequences for antimicrobial resistance gene detection in *E. coli*application/vnd.openxmlformats-officedocument.spreadsheetml.sheetTable_S2.csvMetadata of curated CTX-M *E. coli* reference strain collection from BV-BRC for cgMLST-based phylogenetic comparisontext/csvTable_S2.xlsxMetadata of curated CTX-M *E. coli* reference strain collection from BV-BRC for cgMLST-based phylogenetic comparisonapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheet