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Part 1: Document Description
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Citation |
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Title: |
Phylogenetic dataset and supplementary material for Surján et al. 2023. Vesper bat polyomaviruses in Central Europe |
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Identification Number: |
hdl:21.15109/ARP/99AJTE |
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Distributor: |
ARP |
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Date of Distribution: |
2025-11-12 |
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Version: |
1 |
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Bibliographic Citation: |
Surján, András; Vidovszky, Márton; Gellért, Ákos; Harrach, Balázs, 2025, "Phylogenetic dataset and supplementary material for Surján et al. 2023. Vesper bat polyomaviruses in Central Europe", https://hdl.handle.net/21.15109/ARP/99AJTE, ARP, V1 |
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Citation |
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Title: |
Phylogenetic dataset and supplementary material for Surján et al. 2023. Vesper bat polyomaviruses in Central Europe |
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Identification Number: |
hdl:21.15109/ARP/99AJTE |
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Authoring Entity: |
Surján, András (Veterinary Medical Research Institute) |
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Vidovszky, Márton (Veterinary Medical Research Institute) |
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Gellért, Ákos (Veterinary Medical Research Institute) |
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Harrach, Balázs (Veterinary Medical Research Institute) |
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Distributor: |
ARP |
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Access Authority: |
Surján, András |
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Depositor: |
Surján, András |
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Date of Deposit: |
2025-11-06 |
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Holdings Information: |
https://hdl.handle.net/21.15109/ARP/99AJTE |
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Study Scope |
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Keywords: |
Agricultural Sciences, polyomavirus, novel species, complete genome, bat, phylogenetics, binding site |
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Abstract: |
Polyomaviruses (PyVs) are known to infect a diverse range of vertebrate host species. We report the discovery of PyVs in vesper bats (family Vespertilionidae) from sampling in Central Europe. Seven partial VP1 sequences from different PyVs were detected in samples originating from six distinct vesper bat species. Using a methodology based on conserved segments within the major capsid virus protein 1 (VP1) among known PyVs, the complete genomes of two different novel bat PyVs were determined. The genetic distances of the large T antigen coding sequences from these PyVs compared to previously-described bat PyVs exceeded 15% meriting classification as representatives of two novel PyV species: Alphapolyomavirus epserotinus and Alphapolyomavirus myodaubentonii. Phylogenetic analysis revealed that both belong to the genus Alphapolyomavirus and clustered together with high confidence in clades including other bat alphapolyomaviruses reported from China, South America and Africa. In silico protein modeling of the VP1 subunits and capsid pentamers, and electrostatic surface potential comparison of the pentamers showed significant differences between the reference template (murine polyomavirus) and the novel bat PyVs. An electrostatic potential difference pattern between the two bat VP1 pentamers was also revealed. Disaccharide molecular docking studies showed that the reference template and both bat PyVs possess the typical shallow sialic acid-binding site located between two VP1 subunits, with relevant oligosaccharide-binding affinities. The characterisation of these novel bat PyVs and the reported properties of their capsid proteins will potentially contribute in the elucidation of the conditions creating the host-pathogen restrictions associated with these viruses. |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
First detection and genome analysis of simple nosed bat polyomaviruses in Central Europe |
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Identification Number: |
10.1016/j.meegid.2023.105439 |
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Bibliographic Citation: |
First detection and genome analysis of simple nosed bat polyomaviruses in Central Europe |
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Label: |
FigureS1.tif |
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Text: |
Molecular docking method validation. |
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Notes: |
image/tiff |
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Label: |
FigureS1_Description.txt |
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Text: |
Figure description for supplementary figure 1 (FigureS1). |
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Notes: |
text/plain |
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Label: |
LTAg_eins.fasta |
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Text: |
Multiple alignment of LTAg (Large Tumor Antigen) gene amino acid sequences of polyomaviruses used for phylogenetic analysis, using MAFFT 7.49 with the E-INS-i algorithm. |
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Notes: |
application/octet-stream |
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Label: |
LTAg_seq.fasta |
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Text: |
Raw LTAg (Large Tumor Antigen) gene amino acid sequences of polyomaviruses used for phylogenetic analysis. |
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Notes: |
application/octet-stream |
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LTAg_tree.nwk |
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Phylogenetic tree reconstruction results in Newick format, based on LTAg (Large Tumor Antigen) gene sequences, using PhyML 3.3.1, with LG+G+I model, SPR for topology and SH-like method for branch support. |
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Notes: |
application/octet-stream |
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Label: |
Read_me.txt |
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Text: |
Information file. |
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Notes: |
text/plain |
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Label: |
TableS1.csv |
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Text: |
Primers used for full genome amplification and primer walking. |
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Notes: |
text/csv |