Supplementary data – Salmonella biofilms and essential oils – Seres-Steinbach, 2026 (hdl:21.15109/ARP/ZELTAE)

View:

Part 1: Document Description
Part 2: Study Description
Part 5: Other Study-Related Materials
Entire Codebook

(external link)

Document Description

Citation

Title:

Supplementary data – Salmonella biofilms and essential oils – Seres-Steinbach, 2026

Identification Number:

hdl:21.15109/ARP/ZELTAE

Distributor:

ARP

Date of Distribution:

2026-06-09

Version:

1

Bibliographic Citation:

Seres-Steinbach, Anita; Szabó, Péter; Balázs, Viktória Lilla; Riethmüller, Eszter; Szmolka, Ama; Bányai, Krisztián; Schneider, György, 2026, "Supplementary data – Salmonella biofilms and essential oils – Seres-Steinbach, 2026", https://hdl.handle.net/21.15109/ARP/ZELTAE, ARP, V1

Study Description

Citation

Title:

Supplementary data – Salmonella biofilms and essential oils – Seres-Steinbach, 2026

Identification Number:

hdl:21.15109/ARP/ZELTAE

Authoring Entity:

Seres-Steinbach, Anita (Department of Medical Microbiology, Medical School, University of Pécs)

Szabó, Péter (Department of Geology and Meteorology, Faculty of Sciences, University of Pécs)

Balázs, Viktória Lilla (Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs)

Riethmüller, Eszter (Department of Pharmacognosy, Faculty of Pharmacy, Semmelweiss University)

Szmolka, Ama (Veterinary Medical Research Institute)

Bányai, Krisztián (Department of Medical Biology, Medical School, University of Pécs)

Schneider, György (Department of Medical Microbiology, Medical School, University of Pécs)

Grant Number:

EKÖP-24-1

Grant Number:

RRF-2.3.1-21-2022-00001

Grant Number:

PD 147156

Grant Number:

SA-27/2021

Distributor:

ARP

Access Authority:

Schneider, György

Depositor:

Kaján, Győző László

Date of Deposit:

2026-06-08

Holdings Information:

https://hdl.handle.net/21.15109/ARP/ZELTAE

Study Scope

Keywords:

Agricultural Sciences, Salmonellae, essential oil, biofilm degradation, biofilm formation, biofilm inhibition

Abstract:

The formation of biofilms by Salmonella is of considerable interest to the food production and medical industries. This study investigated the effects of a carrier medium (Luria–Bertani, Mueller–Hinton II, Brain Heart Infusion or chicken meat juice), temperature (14 °C, 23 °C or 37 °C) and surface type (adhesive, non-adhesive or suspension plate) on biofilm formation in 16 different Salmonella isolates belonging to the serovars S. Enteritidis (five isolates), S. Infantis (five isolates) and S. Typhimurium (six isolates). Chicken meat juice was found to have a moderate yet balanced supportive effect, while Mueller–Hinton II (MH-II) medium drastically supported biofilm formation at low temperatures, albeit with significant variation among the isolates. Temperature and medium also affected the antibacterial, biofilm inhibitory and destructive effects of essential oils. At 14 °C and 23 °C, 35% of essential oils exhibited antibacterial activity against Salmonella serovars at a concentration of 0.1%, as determined by the drop plate method. Ajowan, thyme, orange, clove and oregano EOs completely inhibited biofilm formation at a concentration of 0.1%. More than half of the 60 essential oils tested reduced the optical density of mature biofilms (OD: 0.15–0.36) to below 0.05; ajowan, lime, palmarosa, thyme, oregano and clove were the most effective, exhibiting antibacterial, biofilm inhibitory and biofilm destructive effects on all of the investigated Salmonella isolates.

Methodology and Processing

Sources Statement

Data Access

Notes:

<a href="http://creativecommons.org/licenses/by-nc/4.0">CC BY-NC 4.0</a>

Other Study Description Materials

Related Publications

Citation

Title:

Seres-Steinbach A, Szabó P, Balázs Lilla V, Riethmüller E, Szmolka A, Bányai K, Schneider G. Factors Influencing Biofilm Formation of Salmonella spp. and the Biofilm-Degrading Potential of Essential Oils. Foods. 2026; 15(9):1574.

Identification Number:

10.3390/foods15091574

Bibliographic Citation:

Seres-Steinbach A, Szabó P, Balázs Lilla V, Riethmüller E, Szmolka A, Bányai K, Schneider G. Factors Influencing Biofilm Formation of Salmonella spp. and the Biofilm-Degrading Potential of Essential Oils. Foods. 2026; 15(9):1574.

Other Study-Related Materials

Label:

figure_s1.pdf

Text:

SPME‐GC‐MS analysis of the ajowain, clove, lime, palmarosa, oregano and thyme essential oils

Notes:

application/pdf

Other Study-Related Materials

Label:

readme.md

Text:

readme file

Notes:

text/markdown

Other Study-Related Materials

Label:

table_s1.csv

Text:

List of 60 essential oils used in the study with corrected binomial nomenclature and supplier information

Notes:

text/csv

Other Study-Related Materials

Label:

table_s1.pdf

Text:

List of 60 essential oils used in the study with binomial nomenclature and supplier information

Notes:

application/pdf

Other Study-Related Materials

Label:

table_s2.csv

Text:

Raw OD630 values of biofilm formation experiments for 16 Salmonella isolates across all tested conditions

Notes:

text/csv

Other Study-Related Materials

Label:

table_s2.xlsx

Text:

Raw OD630 values of biofilm formation experiments for 16 Salmonella isolates across all tested conditions

Notes:

application/vnd.openxmlformats-officedocument.spreadsheetml.sheet

Other Study-Related Materials

Label:

table_s3.csv

Text:

Raw OD630 values of biofilm inhibition and degradation experiments using 60 essential oils at 0.1% concentration

Notes:

text/csv

Other Study-Related Materials

Label:

table_s3.xlsx

Text:

Raw OD630 values of biofilm inhibition and degradation experiments using 60 essential oils at 0.1% concentration

Notes:

application/vnd.openxmlformats-officedocument.spreadsheetml.sheet