Supplementary data – Hungarian swine CoVs – Máté, 2026

Version 1.1
Máté, Dóra; Varga-Kugler, Renáta; Kaszab, Eszter; Károlyi, Henrik Fülöp; Görföl, Tamás; Kemenesi, Gábor; Igriczi, Barbara; Balka, Gyula; Domán, Marianna; Bálint, Ádám; Zádori, Zoltán; Fehér, Enikő, 2026, "Supplementary data – Hungarian swine CoVs – Máté, 2026", https://hdl.handle.net/21.15109/ARP/GH1AYK, ARP, V1
Learn about Data Citation Standards.
Contact Owner Share
Dataset Metrics
2 Downloads
Description
The rapid evolution of coronaviruses (CoVs) requires researchers to develop specific yet broad-spectrum detection methods to monitor their constant genomic changes. The goal of the present study was to establish a current pan-coronavirus RT-PCR system capable of detecting a wide variety of CoVs and useful for the investigation of virus diversity and host spectrum. For optimization, one-step and two-step nested RT-PCRs with three RT enzymes were examined, amplifying a ~600 bp long product of the RNA-dependent RNA polymerase. As templates, the in vitro transcribed RNA of ten pathogenic CoVs (SARS-CoV, SARS-CoV-2, NL-63, OC43, feline CoV, porcine epidemic diarrhea virus or PEDV, transmissible gastroenteritis virus or TGEV, canine CoV, bat CoV, and infectious bronchitis virus) were applied instead of the often-used DNA standards. A limit of detection of 5–50 copies/reaction was achieved with a random hexamer-primed two-step RT-PCR and a touchdown cycling profile, representing a lower detection limit and higher specificity compared to previously published primer sets. Swine origin pooled samples (n = 121), collected from apparently healthy herds in Hungary, were tested with the novel RT-PCR system. Sequences of porcine respiratory CoV/TGEV and porcine hemagglutinating encephalomyelitis virus were identified in 24 oral fluid and nasal swab pools, demonstrating the circulation of these viruses in this country, as well as the suitability of the new PCR for their detection. The results highlighted the importance of adequate RT enzyme selection and the use of RNase inhibitors in sample preparation and conservation. (2026-02-23)
Subject Agricultural Sciences
Keyword pan-coronavirus, PCR, mammalian, avian, human, swine
Related Publication Máté, D., Varga-Kugler, R., Kaszab, E., Károlyi, H. F., Görföl, T., Kemenesi, G., Igriczi, B., Balka, G., Domán, M., Bálint, Á., Zádori, Z., & Fehér, E. (2026). Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System. Animals, 16(3), 358. doi: 10.3390/ani16030358
License/Data Use Agreement

CC BY-NC 4.0

1 to 2 of 2 Files
RDRP_NA_seq_and_primers.txt
Plain Text - 758.1 KB
Published Feb 23, 2026
1 Download
MD5: 90537a2a5c07f79ad6f76d245a8d2d2a
The primers and coronavirus RNA-dependent RNA polymerase sequences used for primer design.
Data
Preview "RDRP_NA_seq_and_primers.txt"
readme.txt
Plain Text - 3.1 KB
Published Feb 23, 2026
1 Download
MD5: e7fab612f4155f241d2d0965d12fa324
Documentation
Preview "readme.txt"
Edit File

This file has already been deleted (or replaced) in the current version. It may not be edited.

Restrict Access

Restricting limits access to published files. People who want to use the restricted files can request access by default. If you disable request access, you must add information about access to the Terms of Access field.

Learn about restricting files and dataset access in the User Guide.

Enable access request
You must enable request access or add terms of access to restrict file access.
Save Changes
Edit Embargo

The selected file or files have already been published. Contact an administrator to change the embargo date or reason of the file or files.
Delete Files

The file will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Select File(s)

Please select one or more files.

Share Dataset

Share this dataset on your favorite social media networks.

Continue
Dataset Citations

Citations for this dataset are retrieved from Crossref via DataCite using Make Data Count standards. For more information about dataset metrics, please refer to the User Guide.

Sorry, no citations were found.
Restricted Files Selected

The selected file(s) may not be downloaded because you have not been granted access.

You may request access to the restricted file(s) by clicking the Request Access button.

Download Options

The files selected are too large to download as a ZIP.

You can select individual files that are below the 1.9 GB download limit from the files table, or use the Data Access API for programmatic access to the files.

Select File(s)

Please select a file or files to be downloaded.

Restricted Files Selected

The restricted file(s) selected may not be downloaded because you have not been granted access.

Click Continue to download the files you have access to download.

Ineligible Files Selected

Some file(s) cannot be transferred. (They are restricted, embargoed, or not Globus accessible.)

Click Continue to transfer the elligible files.

Restricted Files in the RO-Crate

The file(s) in the RO-Crate may not be downloaded because you have not been granted access.

You may request access to the restricted file(s) by clicking the Request Access button.

Delete Dataset

Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.

Delete Draft Version

Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.

Unpublished Dataset Private URL

Private URL can only be used with unpublished versions of datasets.

Unpublished Dataset Private URL

Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your unpublished dataset.

Delete Files

The file(s) will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Compute

This dataset contains restricted files you may not compute on because you have not been granted access.

Deaccession Dataset

Are you sure you want to deaccession? The selected version(s) will no longer be viewable by the public.

Deaccession Dataset

Are you sure you want to deaccession this dataset? It will no longer be viewable by the public.

Version Differences Details

Please select two versions to view the differences.

Version Differences Details
 
Version:
Last Updated:
Version:
Last Updated:
Select File(s)

Please select a file or files for access request.

Select File(s)

Embargoed files cannot be accessed. Please select an unembargoed file or files for your access request.

Edit Tags

Select existing file tags or create new tags to describe your files. Each file can have more than one tag.

Request Access

  You need to Sign Up or Log In to request access.

Dataset Terms

Please confirm and/or complete the information needed below in order to request access to files in this dataset.

This dataset is made available under the following terms. Please confirm and/or complete the information needed below in order to continue.

Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation shown on the dataset page.

CC BY-NC 4.0

I have read, understood and accept the General Terms of Use of the ARP Data Repository and I take responsibility for compliance with the terms and conditions contained therein.

Preview Guestbook

Upon downloading files the guestbook asks for the following information.

Account Information

Package File Download

Use the Download URL in a Wget command or a download manager to download this package file. Download via web browser is not recommended. User Guide - Downloading a Dataverse Package via URL

https://repo.researchdata.hu/api/access/datafile/

Compute Batch
Clear Batch
Dataset Persistent Identifier Change Compute Batch
Compute Batch
Submit for Review

You will not be able to make changes to this dataset while it is in review.

Publish Dataset

Are you sure you want to republish this dataset?

Select if this is a minor or major version update.

I have read, understood and accept the terms of the Creative Commons Licenses.
Publish Dataset

This dataset cannot be published until Respiratory Bacteriology Dataverse is published by its administrator.

Publish Dataset

This dataset cannot be published until Respiratory Bacteriology Dataverse and Veterinary Medical Research Institute (ÁTKI) are published.

Return to Author

Return this dataset to contributor for modification.