Supplementary data – M. iowae antimicrobial resistance genetic markers – Buni, 2026

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Buni, Dominika; Kovács, Áron Botond; Wehmann, Enikő; Grózner, Dénes; Bányai, Krisztián; Nagy, Eszter Zsófia; Bradbury, Janet; Bottinelli, Marco; Stefani, Elisabetta; Catania, Salvatore; Lysnyansky, Inna; Kovács, László; Gyuranecz, Miklós; Kreizinger, Zsuzsa, 2026, "Supplementary data – M. iowae antimicrobial resistance genetic markers – Buni, 2026", https://hdl.handle.net/21.15109/ARP/QM0ZUO, ARP, V1
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Description
Mycoplasma iowae is an economically significant pathogen that causes reduced hatchability, late embryo mortality and leg deformities, chondrodystrophy and skeletal lesions in poults. While prevention is essential in the control of infection, the appropriate administration of antibiotics may reduce economic losses during outbreaks. As a first step in the exploration of antimicrobial resistance mechanisms in M. iowae, target modification and efflux pump activity were examined in the present study. Point mutations were analyzed in previously described antibiotic binding sites in the whole genome sequences of 99 M. iowae strains. Mismatch amplification mutation assays (MAMAs) were designed and validated for the differentiation of mutations corresponding to elevated minimum inhibitory concentration (MIC) values for fluoroquinolones. Broth microdilution assays were performed to evaluate the effect of efflux pump inhibitors. In the presence of orthovanadate (OV), MIC values were significantly lower than in the absence of OV for spiramycin, tilmicosin, tylosin and oxytetracycline, which may indicate the presence of an active efflux system in M. iowae. Putative promoter regions of efflux-related genes were predicted and characterized. Genetic mutations, previously described in other bacteria, were described to be associated with elevated fluoroquinolone, macrolide and lincomycin MICs in M. iowae, although certain resistant phenotypes remained unexplained, promoting future examinations for deeper insights. The developed MAMAs may support rapid identification of M. iowae strains with elevated MIC values for fluoroquinolones. The better understanding of the efflux pump mechanisms enables the development of alternative methods for the support of therapy against this pathogen. (2026-06-11)
Subject Agricultural Sciences
Keyword Mycoplasma iowae, Antimicrobial resistance, Efflux pumps, Mismatch amplification mutation assay (MAMA), Orthovanadate, Turkey pathogen
Related Publication Is Supplement To: Identification and detection of genetic markers associated with antimicrobial susceptibility and evaluation of efflux pump mechanisms in Mycoplasma iowae Buni D, Kovács ÁB, Wehmann E, Grózner D, Bányai K, et al. (2026) Identification and detection of genetic markers associated with antimicrobial susceptibility and evaluation of efflux pump mechanisms in Mycoplasma iowae. PLOS ONE 21(4): e0347345doi: 10.1371/journal.pone.0347345
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readme.md
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Published Jun 12, 2026
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table_s1.xlsx
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Published Jun 12, 2026
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MD5: ce4a13bcd8e42de95ac7031034f4f3b9
This table contains information on 99 *M. iowae* clinical isolates and strains, including strain designation, country of origin, host species, serotype, minimum inhibitory concentrations (MIC) for enrofloxacin, erythromycin, spiramycin, tilmicosin, tylosin, and lincomycin. It also lists the non-synonymous single nucleotide polymorphisms (nsSNPs) identified in the *gyrA*, *parC* and 23S rRNA genes associated with antimicrobial resistance.
table_s1_sheet_1.csv
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Published Jun 12, 2026
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MD5: b349a17817525003870a3626ec5972f4
This table contains information on 99 *M. iowae* clinical isolates and strains, including strain designation, country of origin, host species, serotype, and minimum inhibitory concentrations (MIC) for enrofloxacin. It also lists the non-synonymous single nucleotide polymorphisms (nsSNPs) identified in the *gyrA* and *parC* genes associated with antimicrobial resistance.
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table_s1_sheet_2.csv
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Published Jun 12, 2026
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MD5: cf146315c8f56802bace74f39930df13
This table contains information on 99 *M. iowae* clinical isolates and strains, including strain designation, country of origin, host species, serotype, minimum inhibitory concentrations (MIC) for erythromycin, spiramycin, tilmicosin, tylosin, and lincomycin. It also lists the non-synonymous single nucleotide polymorphisms (nsSNPs) identified in the 23S rRNA genes associated with antimicrobial resistance.
Preview "table_s1_sheet_2.csv"
table_s2.csv
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Published Jun 12, 2026
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MD5: e5081344cd0760b3e1daae8d238d8879
This table presents non-synonymous SNPs identified in putative efflux pump-related genes in *M. iowae* strains used for efflux pump investigation. It includes the MIC values of enrofloxacin, lincomycin, oxytetracycline, spiramycin, tilmicosin and tylosin measured both in the absence and presence of sodium orthovanadate (OV). The data supports the evaluation of efflux pump-mediated antimicrobial resistance mechanisms.
Preview "table_s2.csv"
table_s2.xlsx
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Published Jun 12, 2026
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MD5: 546b7374a3c5492f6c0612702be278cf
This table presents non-synonymous SNPs identified in putative efflux pump-related genes in *M. iowae* strains used for efflux pump investigation. It includes the MIC values of enrofloxacin, lincomycin, oxytetracycline, spiramycin, tilmicosin and tylosin measured both in the absence and presence of sodium orthovanadate (OV). The data supports the evaluation of efflux pump-mediated antimicrobial resistance mechanisms.
table_s3.csv
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MD5: abd47d883771bae39245b0316189daf6
Data used for logistic regression and Fisher's exact test to analyze associations among MIC values and genotypes in *Mycoplasma iowae*.
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table_s3.xlsx
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Published Jun 12, 2026
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MD5: 12eab1525483f9a708335c63b56af726
Data used for logistic regression and Fisher's exact test to analyze associations among MIC values and genotypes in *Mycoplasma iowae*.
table_s4.csv
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MD5: 7e99e5636b0acb8c0c5dea50d05d4369
Data used for Wilcoxon Signed-Rank test to analyze the effect of sodium orthovanadate on MIC values against *Mycoplasma iowae* strains.
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table_s4.xlsx
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Published Jun 12, 2026
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MD5: 9479c55b8506f36a301cc364234b70e9
Data used for Wilcoxon Signed-Rank test to analyze the effect of sodium orthovanadate on MIC values against *Mycoplasma iowae* strains.
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